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CHRNA7 antibody - N-terminal region
Rabbit Polyclonal Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB |
|---|---|
| Primary Accession | P36544 |
| Other Accession | NM_000746, NP_000737 |
| Reactivity | Human, Mouse, Rat, Bovine |
| Predicted | Human, Mouse, Rat, Chicken, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 56449 Da |
| Gene ID | 1139;89832 |
|---|---|
| Alias Symbol | NACHRA7, CHRNA7-2 |
| Other Names | Neuronal acetylcholine receptor subunit alpha-7, CHRNA7, NACHRA7 |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 100 ul of distilled water. Final anti-CHRNA7 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | CHRNA7 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CHRNA7 (HGNC:1960) |
|---|---|
| Synonyms | NACHRA7 |
| Function | Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (PubMed:15609996, PubMed:33735609, PubMed:8145738). CHRNA7 forms homopentameric neuronal acetylcholine receptors abundantly expressed in the central nervous system, characterized by fast desensitization and high calcium permeability (PubMed:31560909, PubMed:33735609, PubMed:38382524, PubMed:8145738). Also forms heteropentamers with CHRNB2, mainly expressed in basal forebrain cholinergic neurons. Involved in the modulation of calcium- dependent signaling pathways and influences the release of neurotransmitters, including dopamine, glutamate and GABA (PubMed:33239400). Also expressed in non-neuronal cells such as immune cells like lymphocytes, monocytes and macrophages (PubMed:12508119, PubMed:16968406, PubMed:25259522). In T cells, activation induces metabotropic signaling that results in an increase of intracellular Ca2+ concentrations, independent of ionotropic receptor functions (PubMed:17709503). In macrophages, required for acetylcholine-mediated inhibition of TNF and other inflammatory cytokine release (PubMed:12508119). Once activated by acetylcholine, nicotine or other agonists, selectively inhibits production of pro-inflammatory cytokines while leaving anti-inflammatory cytokines undisturbed (PubMed:12508119, PubMed:25259522). Stimulates the cholinergic anti-inflammatory pathway, controlling inflammation by inhibiting NFKB nuclear translocation and activating the JAK2-STAT3 pathway, independently of ion channel activity (PubMed:16968406, PubMed:25259522). Also expressed in the urothelium where it modulates reflex bladder activity by increasing intracellular calcium through internal stores and decreasing basal ATP release (By similarity). |
| Cellular Location | Postsynaptic cell membrane {ECO:0000250|UniProtKB:Q05941}; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=TMEM35A/NACHO promotes its trafficking to the cell membrane (PubMed:27789755). RIC3 promotes its trafficking to the cell membrane (By similarity) {ECO:0000250|UniProtKB:Q05941, ECO:0000269|PubMed:27789755} |
| Tissue Location | Expressed in neuronal cells (PubMed:8145738). Expressed in macrophages (at protein level) (PubMed:12508119) |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
REFERENCES
Peng X.,et al.Mol. Pharmacol. 45:546-554(1994).
Logel J.,et al.Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
Elliott K.J.,et al.J. Mol. Neurosci. 7:217-228(1996).
Groot Kormelink P.J.,et al.FEBS Lett. 400:309-314(1997).
Groot Kormelink P.J.,et al.Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
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