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>   首页   >   产品   >   一抗   >   精选抗体   >   细胞自噬抗体   >   LC3 Antibody (APG8)   

LC3 Antibody (APG8)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - LC3 Antibody (APG8) AM1800A
    All lanes : Anti-MAP1LC3A Antibody at dilution Lane 1: Mouse brain whole cell lysate Lane 2: Rat brain whole cell lysate Lane 3: Human brain whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - LC3 Antibody (APG8) AM1800A
    Western blot analysis of anti-LC3 Mab (Cat. #AM1800a) at 8 μg/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).
  • 1 - LC3 Antibody (APG8) AM1800A
    Western blot analysis of anti-LC3 Mab (Cat. #AM1800a) Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9H492, Q9GZQ8
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1 k
Clone Names 166AT1234
Calculated MW 14272 Da
Additional Information
Gene ID 84557
Other Names Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A
Target/Specificity This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLC3 Antibody (APG8) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP1LC3A
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538, PubMed:31006537).
Cellular Location Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Endomembrane system; Lipid-anchor. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q91VR7}. Note=LC3-II binds to the autophagic membranes.
Tissue Location Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes
Research Areas

BACKGROUND

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

REFERENCES

References for protein:
1.Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation. Gao C, et al. Nat Cell Biol, 2010 Aug. PMID 20639871.
2.The prolyl isomerase Pin1 induces LC-3 expression and mediates tamoxifen resistance in breast cancer. Namgoong GM, et al. J Biol Chem, 2010 Jul 30. PMID 20479004.
3.Protein kinase C inhibits autophagy and phosphorylates LC3. Jiang H, et al. Biochem Biophys Res Commun, 2010 May 14. PMID 20398630.
4. Processing of autophagic protein LC3 by the 20S proteasome. Gao Z, et al. Autophagy, 2010 Jan. PMID 20061800.
5.Defective autophagy associated with LC3 puncta in epothilone-resistant cancer cells. Shen S, et al. Cell Cycle, 2010 Jan 15. PMID 20023420.
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].

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