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Cleaved LC3A Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - Cleaved LC3A Antibody AP1805a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 ( Mouse mouse embryonic fibroblasts cell line) cells labeling MAP1LC3A with AP1805A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). The nuclear counter stain is DAPI (blue). Immunofluorescence image showing cytoplasm on NIH/3T3 cell line.
  • 3 - Cleaved LC3A Antibody AP1805a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling MAP1LC3A with AP1805A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Immunofluorescence image showing vesicles staining on Hela cell line.The nuclear counter stain is DAPI (blue).The right image is Hela cells treated with Chloroquine 50μM for 16h.
  • 14 - Cleaved LC3A Antibody AP1805a
    AP1805A staining Cleaved-APG8a in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - Cleaved LC3A Antibody AP1805a
    Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A) Antibody (Cat. #AP1805a)(upper) or Beta-actin (lower).
  • 1 - Cleaved LC3A Antibody AP1805a
    Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-APG8a (MAP1LC3A)(RB52604)(upper) or Beta-actin (lower).
  • 1 - Cleaved LC3A Antibody AP1805a
    Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-APG8a (MAP1LC3A)(Cat. #AP1805a)(upper) or Beta-actin (lower).
  • 1 - Cleaved LC3A Antibody AP1805a
    Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A)(Cat. #AP1805a)(upper) or Beta-actin (lower).
  • 1 - Cleaved LC3A Antibody AP1805a
    Western blot analysis of anti-cleaved-LC3 (APG8a) Pab (Cat. #AP1805a) in mouse brain tissue lysate. Cleaved-LC3 (APG8a) was detected using the purified Pab.
  • 14 - Cleaved LC3A Antibody AP1805a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 8 - Cleaved LC3A Antibody AP1805a
    5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in 4% of paraformaldehyde. Treatment with Cat# AP1805a antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
  • 产品详情
  • 文献引用 : 39
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, ICC, IF, E
Primary Accession Q9H492, Q9GZQ8
Other Accession Q62625, Q9CQV6, O41515, Q6XVN8, Q91VR7, Q2HJ23
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Other Names Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A
Target/Specificity This Cleaved LC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-120 amino acids from human Cleaved LC3A.
Dilution IF~~1:25
IHC-P~~1:10~50
WB~~1:1000
ICC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCleaved LC3A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Tumor autophagy is associated with survival outcomes in patients with resected non-small cell lung cancer.
Author : Überall I1,Gachechiladze M2,Joerger M3,Anděl J4,Smičková P5,Kolek V5,Grygárková I5,Škarda J6.
Lung Cancer. 2019 Mar;129:85-91. doi: 10.1016/j.lungcan.2019.01.001. Epub 2019 Jan 8.
30797498
Three-Dimensional Organoids Reveal Therapy Resistance of Esophageal and Oropharyngeal Squamous Cell Carcinoma Cells.
Author : Kijima T1,Nakagawa H2,Shimonosono M3,Chandramouleeswaran PM4,Hara T5,Sahu V6,Kasagi Y7,Kikuchi O8,Tanaka K9,Giroux V4,Muir AB7,Whelan KA10,Ohashi S11,Naganuma S12,Klein-Szanto AJ13,Shinden Y14,Sasaki K14,Omoto I14,Kita Y14,Muto M11,Bass AJ15,Diehl JA16,Gi
Cell Mol Gastroenterol Hepatol. 2018 Sep 14;7(1):73-91. doi: 10.1016/j.jcmgh.2018.09.003. eCollection 2019.
30510992
Inhibition of excessive autophagy and mitophagy mediates neuroprotective effects of URB597 against chronic cerebral hypoperfusion.
Author : Su SH1,Wu YF2,Wang DP2,Hai J3.
Cell Death Dis. 2018 Jun 28;9(7):733. doi: 10.1038/s41419-018-0755-y.
29955058
Autophagy dysregulation caused by ApoM deficiency plays an important role in liver lipid metabolic disorder.
Author : Zhang X1,Zhang P2,Gao J3,Huang Q4.
Biochem Biophys Res Commun. 2017 Dec 27. pii: S0006-291X(17)32550-0. doi: 10.1016/j.bbrc.2017.12.148. [Epub ahead of print]
29288662
Cysteamine-mediated clearance of antibiotic-resistant pathogens in human cystic fibrosis macrophages.
Author : Shrestha CL1,Assani KD1,Rinehardt H1,Albastroiu F1,Zhang S1,Shell R2,Amer AO3,4,Schlesinger LS3,Kopp BT1,2,3.
PLoS One. 2017 Oct 5;12(10):e0186169. doi: 10.1371/journal.pone.0186169. eCollection 2017.
28982193
Three-dimensional tumor cell growth stimulates autophagic flux and recapitulates chemotherapy resistance.
Author : Bingel C1,Koeneke E1,2,Ridinger J1,2,Bittmann A1,2,Sill M3,Peterziel H1,2,Wrobel JK1,2,Rettig I1,Milde T1,2,4,Fernekorn U5,Weise F5,Schober A5,Witt O1,2,Oehme I1,2.
Cell Death Dis. 2017 Aug 24;8(8):e3013. doi: 10.1038/cddis.2017.398.
28837150
Standard Immunohistochemical Assays to Assess Autophagy in Mammalian Tissue.
Author : Martinet W1,Roth L2,De Meyer GRY3.
Cells. 2017 Jun 30;6(3). pii: E17. doi: 10.3390/cells6030017.
28665306
Vitamin D receptor regulates autophagy in the normal mammary gland and in luminal breast cancer cells.
Author : Tavera-Mendoza LE1,2,Westerling T1,2,Libby E3,Marusyk A4,Cato L1,2,Cassani R5,Cameron LA6,Ficarro SB7,Marto JA7,Klawitter J8,Brown M9,2.
Proc Natl Acad Sci U S A. 2017 Feb 27. pii: 201615015. doi: 10.1073/pnas.1615015114. [Epub ahead of print]
28242709
Inhibition of autophagy protein LC3A as a therapeutic target in ovarian clear cell carcinomas.
Author : Miyamoto M1,Takano M2,Aoyama T1,Soyama H1,Yoshikawa T3,Tsuda H4,Furuya K1.
J Gynecol Oncol. 2017 Feb 13. [Epub ahead of print]
28382796
Transcription Factor EB Expression in Early Breast Cancer Relates to Lysosomal/Autophagosomal Markers and Prognosis.
Author : Giatromanolaki A1,Sivridis E1,Kalamida D2,Koukourakis MI3.
Clin Breast Cancer. 2016 Nov 23. pii: S1526-8209(16)30497-9. doi: 10.1016/j.clbc.2016.11.006. [Epub ahead of print]
28017540
ALDH2 modulates autophagy flux to regulate acetaldehyde-mediated toxicity thresholds.
Author : Tanaka K1, Whelan KA1, Chandramouleeswaran PM1, Kagawa S1, Rustgi SL1, Noguchi C2, Guha M3, Srinivasan S3, Amanuma Y4, Ohashi S4, Muto M4 Am J Cancer Res. 2016 Mar 15;6(4):781-96. eCollection 2016.
27186430
Increased expression of transcription factor EB (TFEB) is associated with autophagy, migratory phenotype and poor prognosis in non-small cell lung cancer.
Author : Giatromanolaki A1, Kalamida D2, Sivridis E1, Karagounis IV2, Gatter KC3, Harris AL4, Koukourakis MI5.
Lung Cancer. 2015 Oct;90(1):98-105. doi: 10.1016/j.lungcan.2015.07.008. Epub 2015 Jul 26.
26264650
Autophagy levels are elevated in barrett\'s esophagus and promote cell survival from acid and oxidative stress.
Author : Kong J1, Whelan KA1, Laczkó D1, Dang B1, Caro Monroig A1, Soroush A1, Falcone J1, Amaravadi RK2,3, Rustgi AK1, Ginsberg GG1, Falk GW Mol Carcinog. 2015 Sep 16. doi: 10.1002/mc.22406. [Epub ahead of print]
26373456
Particulate cytoplasmic structures with high concentration of ubiquitin-proteasome accumulate in myeloid neoplasms.
Author : Pecci A1, Necchi V2,3, Barozzi S4, Vitali A5, Boveri E6, Elena C7, Bernasconi P8, Noris P9, Solcia E10,11.
J Hematol Oncol. 2015 Jun 18;8(1):71.
26081257
Chaperone molecules concentrate together with the ubiquitin-proteasome system inside particulate cytoplasmic structures: possible role in metabolism of misfolded proteins.
Author : Vanoli A1, Necchi V, Barozzi S, Manca R, Pecci A, Solcia E.
Histochem Cell Biol. 2015 May 8. [Epub ahead of print]
25952156
Mineralocorticoid receptor antagonism induces browning of white adipose tissue through impairment of autophagy and prevents adipocyte dysfunction in high-fat-diet-fed mice.
Author : Armani A1, Cinti F2, Marzolla V1, Morgan J3, Cranston GA3, Antelmi A1, Carpinelli G4, Canese R4, Pagotto U5, Quarta C5, Malorni W6, Ma
FASEB J. 2014 Aug;28(8):3745-57. doi: 10.1096/fj.13-245415. Epub 2014 May 7.
24806198
IFN-γ Stimulates Autophagy-Mediated Clearance of Burkholderia cenocepacia in Human Cystic Fibrosis Macrophages.
Author : Assani K1, Tazi MF2, Amer AO2, Kopp BT3.
PLoS One. 2014 May 5;9(5):e96681. doi: 10.1371/journal.pone.0096681. eCollection 2014.
24798083
LC3A-positive
Author : Liao W1,Sun L,Wang C,Huang H,Liu J,Liao W,Shi M.
Anat Rec (Hoboken). 2014 Apr;297(4):653-62. doi: 10.1002/ar.22895. Epub 2014 Feb 14.
24532538
Autophagy proteins in prostate cancer: Relation with anaerobic metabolism and Gleason score.
Author : Giatromanolaki A1, Sivridis E2, Mendrinos S3, Koutsopoulos AV2, Koukourakis MI4.
Urol Oncol. 2014 Jan;32(1):39.e11-8. doi: 10.1016/j.urolonc.2013.04.003. Epub 2013 Jun 17.
23787295
Patterns of autophagy in urothelial cell carcinomas--the significance of "stone-like" structures (SLS) in transurethral resection biopsies.
Author : Sivridis E, Koukourakis MI, Mendrinos SE, Touloupidis S, Giatromanolaki A.
Urol Oncol. 2013 Oct;31(7):1254-60. doi: 10.1016/j.urolonc.2011.12.016. Epub 2012 Jan 24.
22281431
Autophagy and Bcl-2/BNIP3 death regulatory pathway in non-small cell lung carcinomas.
Author : Karpathiou G, Sivridis E, Koukourakis M, Mikroulis D, Bouros D, Froudarakis M, Bougioukas G, Maltezos E, Giatromanolaki A.
APMIS. 2013 Jul;121(7):592-604. doi: 10.1111/apm.12026. Epub 2012 Dec 8.
23216071
LC3 immunostaining pitfalls.
Author : Koukourakis MI, Giatromanolaki A, Zois CE, Sivridis E.
Histopathology. 2013 May;62(6):962-3. doi: 10.1111/his.12080. Epub 2013 Mar 26.
23530618
A bacterial protein promotes the recognition of the Legionella pneumophila vacuole by autophagy.
Author : Khweek AA, Caution K, Akhter A, Abdulrahman BA, Tazi M, Hassan H, Majumdar N, Doran A, Guirado E, Schlesinger LS, Shuman H, Amer AO.
Eur J Immunol. 2013 May;43(5):1333-44. doi: 10.1002/eji.201242835. Epub 2013 Apr 8.
23420491
Inhibition of the host translation shutoff response by herpes simplex virus 1 triggers nuclear envelope-derived autophagy.
Author : Radtke K, English L, Rondeau C, Leib D, Lippé R, Desjardins M.
J Virol. 2013 Apr;87(7):3990-7. doi: 10.1128/JVI.02974-12. Epub 2013 Jan 30.
23365427
Overexpression of LC3A autophagy protein in follicular and diffuse large B-cell lymphomas.
Author : Giatromanolaki A, Koukourakis MI, Pouliliou S, Gatter KC, Pezzella F, Harris AL, Sivridis E.
Hematol Oncol Stem Cell Ther. 2013 Mar;6(1):20-5. doi: 10.1016/j.hemonc.2013.02.001. Epub 2013 Feb 28.
23664601
Immunohistochemical analysis of macroautophagy: recommendations and limitations.
Author : Martinet W, Schrijvers DM, Timmermans JP, Bult H, De Meyer GR.
Autophagy. 2013 Mar;9(3):386-402. doi: 10.4161/auto.22968. Epub 2012 Dec 14.
23242143
Depletion of the ubiquitin-binding adaptor molecule SQSTM1/p62 from macrophages harboring cftr ΔF508 mutation improves the delivery of Burkholderia cenocepacia to the autophagic machinery.
Author : Abdulrahman BA, Khweek AA, Akhter A, Caution K, Tazi M, Hassan H, Zhang Y, Rowland PD, Malhotra S, Aeffner F, Davis IC, Valvano MA, Amer AO.
J Biol Chem. 2013 Jan 18;288(3):2049-58. doi: 10.1074/jbc.M112.411728. Epub 2012 Nov 12.
23148214
Low expression of ULK1 is associated with operable breast cancer progression and is an adverse prognostic marker of survival for patients.
Author : Tang J, Deng R, Luo RZ, Shen GP, Cai MY, Du ZM, Jiang S, Yang MT, Fu JH, Zhu XF.
Breast Cancer Res Treat. 2012 Jul;134(2):549-60. doi: 10.1007/s10549-012-2080-y. Epub 2012 May 15.
22585231
The gluttonous side of malignant melanoma: basic and clinical implications of macroautophagy.
Author : Checinska A, Soengas MS.
Pigment Cell Melanoma Res. 2011 Dec;24(6):1116-32. doi: 10.1111/j.1755-148X.2011.00927.x.
21995431
"Autophagic flux" in normal mouse tissues: focus on endogenous LC3A processing.
Author : Zois CE, Giatromanolaki A, Sivridis E, Papaiakovou M, Kainulainen H, Koukourakis MI.
Autophagy. 2011 Nov;7(11):1371-8. doi: 10.4161/auto.7.11.16664. Epub 2011 Nov 1.
21997374
Autophagy stimulation by rapamycin suppresses lung inflammation and infection by Burkholderia cenocepacia in a model of cystic fibrosis.
Author : Abdulrahman BA, Khweek AA, Akhter A, Caution K, Kotrange S, Abdelaziz DH, Newland C, Rosales-Reyes R, Kopp B, McCoy K, Montione R, Schlesinger LS, Gavrilin MA, Wewers MD, Valvano MA, Amer AO.
Autophagy. 2011 Nov;7(11):1359-70. doi: 10.4161/auto.7.11.17660. Epub 2011 Nov 1.
21997369
Autophagy patterns and prognosis in uveal melanomas.
Author : Giatromanolaki AN, Charitoudis GS, Bechrakis NE, Kozobolis VP, Koukourakis MI, Foerster MH, Sivridis EL.
Mod Pathol. 2011 Aug;24(8):1036-45. doi: 10.1038/modpathol.2011.63. Epub 2011 Apr 15.
21499230
Beclin-1 and LC3A expression in cutaneous malignant melanomas: a biphasic survival pattern for beclin-1.
Author : Sivridis E, Koukourakis MI, Mendrinos SE, Karpouzis A, Fiska A, Kouskoukis C, Giatromanolaki A.
Melanoma Res. 2011 Jun;21(3):188-95. doi: 10.1097/CMR.0b013e328346612c.
21537144
LC3A-positive "stone-like" structures in cutaneous squamous cell carcinomas.
Author : Sivridis E, Giatromanolaki A, Karpathiou G, Karpouzis A, Kouskoukis C, Koukourakis MI.
Am J Dermatopathol. 2011 May;33(3):285-90. doi: 10.1097/DAD.0b013e3181f10de0.
21430508
Pancreatic cancers require autophagy for tumor growth.
Author : Yang S, Wang X, Contino G, Liesa M, Sahin E, Ying H, Bause A, Li Y, Stommel JM, Dell'antonio G, Mautner J, Tonon G, Haigis M, Shirihai OS, Doglioni C, Bardeesy N, Kimmelman AC.
Genes Dev. 2011 Apr 1;25(7):717-29. doi: 10.1101/gad.2016111. Epub 2011 Mar 15.
21406549
Lung autophagic response following exposure of mice to whole body irradiation, with and without amifostine.
Author : Zois CE, Giatromanolaki A, Kainulainen H, Botaitis S, Torvinen S, Simopoulos C, Kortsaris A, Sivridis E, Koukourakis MI.
Biochem Biophys Res Commun. 2011 Jan 7;404(1):552-8. doi: 10.1016/j.bbrc.2010.12.024. Epub 2010 Dec 8.
21145309
Autophagy in endometrial carcinomas and prognostic relevance of 'stone-like' structures (SLS): what is destined for the atypical endometrial hyperplasia?
Author : Sivridis E, Giatromanolaki A, Liberis V, Koukourakis MI.
Autophagy. 2011 Jan;7(1):74-82. Epub 2011 Jan 1.
21099253
Prognostic relevance of light chain 3 (LC3A) autophagy patterns in colorectal adenocarcinomas.
Author : Giatromanolaki A, Koukourakis MI, Harris AL, Polychronidis A, Gatter KC, Sivridis E.
J Clin Pathol. 2010 Oct;63(10):867-72. doi: 10.1136/jcp.2010.079525.
20876316
LC3A-positive light microscopy detected patterns of autophagy and prognosis in operable breast carcinomas.
Author : Sivridis E, Koukourakis MI, Zois CE, Ledaki I, Ferguson DJ, Harris AL, Gatter KC, Giatromanolaki A.
Am J Pathol. 2010 May;176(5):2477-89. doi: 10.2353/ajpath.2010.090049. Epub 2010 Apr 9.
20382705

BACKGROUND

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

REFERENCES

References for protein:
1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
3.Greenberg JT. Dev Cell. 8(6):799-801. (2005)
4. Levine B. Cell. 120(2):159-62. (2005)
5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
6.Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004)
7.He H., et al. J. Biol. Chem. 278:29278-29287(2003)
8.Tanida I., et al. J. Biol. Chem. 279:36268-36276(2004)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].

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