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Cleaved LC3A Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - Cleaved LC3A Antibody AP1805a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 ( Mouse mouse embryonic fibroblasts cell line) cells labeling MAP1LC3A with AP1805A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). The nuclear counter stain is DAPI (blue). Immunofluorescence image showing cytoplasm on NIH/3T3 cell line.
  • 3 - Cleaved LC3A Antibody AP1805a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling MAP1LC3A with AP1805A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Immunofluorescence image showing vesicles staining on Hela cell line.The nuclear counter stain is DAPI (blue).The right image is Hela cells treated with Chloroquine 50μM for 16h.
  • 14 - Cleaved LC3A Antibody AP1805a
    AP1805A staining Cleaved-APG8a in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - Cleaved LC3A Antibody AP1805a
    Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A) Antibody (Cat. #AP1805a)(upper) or Beta-actin (lower).
  • 1 - Cleaved LC3A Antibody AP1805a
    Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-APG8a (MAP1LC3A)(RB52604)(upper) or Beta-actin (lower).
  • 1 - Cleaved LC3A Antibody AP1805a
    Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-APG8a (MAP1LC3A)(Cat. #AP1805a)(upper) or Beta-actin (lower).
  • 1 - Cleaved LC3A Antibody AP1805a
    Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A)(Cat. #AP1805a)(upper) or Beta-actin (lower).
  • 1 - Cleaved LC3A Antibody AP1805a
    Western blot analysis of anti-cleaved-LC3 (APG8a) Pab (Cat. #AP1805a) in mouse brain tissue lysate. Cleaved-LC3 (APG8a) was detected using the purified Pab.
  • 14 - Cleaved LC3A Antibody AP1805a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 8 - Cleaved LC3A Antibody AP1805a
    5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in 4% of paraformaldehyde. Treatment with Cat# AP1805a antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
  • 产品详情
  • 文献引用 : 43
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, ICC, IF, E
Primary Accession Q9H492, Q9GZQ8
Other Accession Q62625, Q9CQV6, O41515, Q6XVN8, Q91VR7, Q2HJ23
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Additional info
Other Names Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A
Target/Specificity This Cleaved LC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-120 amino acids from human Cleaved LC3A or LC3B.
Dilution IF~~1:25
IHC-P~~1:10~50
WB~~1:1000
ICC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCleaved LC3A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas

BACKGROUND

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

REFERENCES

References for protein:
1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
3.Greenberg JT. Dev Cell. 8(6):799-801. (2005)
4. Levine B. Cell. 120(2):159-62. (2005)
5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
6.Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004)
7.He H., et al. J. Biol. Chem. 278:29278-29287(2003)
8.Tanida I., et al. J. Biol. Chem. 279:36268-36276(2004)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].

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