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PPARA Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - PPARA Antibody AM8425b
    All lanes: Anti-PPARA Antibody at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: U-87 MG whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated (ASP1613) at 1/8000 dilution. Observed band size: 52 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - PPARA Antibody AM8425b
    All lanes: Anti-PPARA Antibody at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: C2C12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-mouse IgG, (H+L), Peroxidase conjugated (ASP1613) at 1/15000 dilution. Observed band size: 52KDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - PPARA Antibody AM8425b
    Flow cytometric analysis of Hela cells using PPARA Antibody(green, Cat#AM8425b) compared to an isotype control of mouse IgG1(blue). AM8425b was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
  • 3 - PPARA Antibody AM8425b
    Fluorescent image of Hela cells stained with PPARA Antibody(Cat#AM8425b). AM8425b was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - PPARA Antibody AM8425b
    Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using PPARA Antibody(Cat#AM8425b). AM8425b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, FC, E
Primary Accession Q07869
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1,κ
Clone Names 1331CT894.186.143
Calculated MW 52225 Da
Antigen Region 1-468 aa
Additional Information
Gene ID 5465
Other Names Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, PPARA, NR1C1, PPAR
Target/Specificity This PPARA antibody is generated from a mouse immunized with a recombination protein from the human region of human PPARA.
Dilution WB~~1:1000
IHC-P~~1:100~500
IF~~1:25
FC~~1:25
E~~Use at an assay dependent concentration.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPARA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPARA
Synonyms NR1C1, PPAR
Function Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn- glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
Cellular Location Nucleus.
Tissue Location Skeletal muscle, liver, heart and kidney. Expressed in monocytes (PubMed:28167758).
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.

REFERENCES

Sher T.,et al.Biochemistry 32:5598-5604(1993).
Mukherjee R.,et al.J. Steroid Biochem. Mol. Biol. 51:157-166(1994).
Tugwood J.D.,et al.Ann. N. Y. Acad. Sci. 804:252-265(1996).
Kobayashi T.,et al.FEBS Lett. 582:2737-2744(2008).
Cho M.-C.,et al.Immunopharmacol. Immunotoxicol. 31:459-467(2009).

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