RAB5C Antibody

Purified Mouse Monoclonal Antibody (Mab)

标注"皇冠"的产品，是满足高质量标准的产品。
了解更多...

Overlay histogram showing U-2OS cells stained with AM8520b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8520b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-RAB5C Antibody at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	P51148
Other Accession	Q5R7L7
Reactivity	Human, Mouse
Host	Mouse
Clonality	monoclonal
Isotype	IgG2b,k
Clone Names	1616CT314.65.31
Calculated MW	23483 Da

Additional Information
Gene ID	5878
Other Names	Ras-related protein Rab-5C, L1880, RAB5L, RAB5C, RABL
Target/Specificity	This RAB5C antibody is generated from a mouse immunized with a recombinant protein of human RAB5C.
Dilution	WB~~1:2000 FC~~1:25 E~~Use at an assay dependent concentration.
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RAB5C Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	RAB5C (HGNC:9785)
Synonyms	RABL
Function	The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:P20339}; Lipid-anchor {ECO:0000250\|UniProtKB:P20339}; Cytoplasmic side {ECO:0000250\|UniProtKB:P20339}. Early endosome membrane {ECO:0000250\|UniProtKB:P20339}; Lipid-anchor {ECO:0000250\|UniProtKB:P20339}. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Protein transport. Probably involved in vesicular traffic (By similarity).

REFERENCES

Han H.J.,et al.Cytogenet. Cell Genet. 73:137-139(1996).
Albertsen H.M.,et al.Nat. Genet. 7:472-479(1994).
Clemens D.L.,et al.Infect. Immun. 68:2671-2684(2000).
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问，联系：0512-88856768 tech-china@abcepta.com.

我要评价

¥ 1,250.00

Cat# AM8520b

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

Quantity:

reduce add

Availability: In Stock

询价

RAB5C Antibody

Purified Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

相关产品

多肽合成服务

多克隆抗体制备服务

单克隆抗体制备服务

抗体测序服务

蛋白表达服务

药物发现定制服务