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ME2 Antibody (C-term)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 14 - ME2 Antibody (C-term) AM8621b
    AM8621b staining ME2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - ME2 Antibody (C-term) AM8621b
    All lanes : Anti-ME2 Antibody (C-term) at 1:4000 dilution Lane 1: HL-60 whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P23368
Reactivity Human, Mouse
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 970CT2.1.1.2
Calculated MW 65444 Da
Additional Information
Gene ID 4200
Other Names NAD-dependent malic enzyme, mitochondrial, NAD-ME, 1.1.1.38, Malic enzyme 2, ME2
Target/Specificity This ME2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 522-556 amino acids from the C-terminal region of human ME2.
Dilution WB~~1:4000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsME2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ME2
Function NAD-dependent mitochondrial malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate.
Cellular Location Mitochondrion matrix
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

Loeber G.,et al.J. Biol. Chem. 266:3016-3021(1991).
Yanaihara N.,et al.Genomics 72:169-179(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Nusbaum C.,et al.Nature 437:551-555(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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