癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
CYP27B1 Antibody (C-term)
Purified Mouse Monoclonal Antibody (Mab)
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- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | O15528 |
| Reactivity | Human, Rat, Mouse |
| Predicted | Human |
| Host | Mouse |
| Clonality | monoclonal |
| Isotype | IgG1,κ |
| Clone Names | 1981CT820.303.81 |
| Calculated MW | 56504 Da |
| Gene ID | 1594 |
|---|---|
| Other Names | 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, 1.14.13.13, 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, VD3 1A hydroxylase, Calcidiol 1-monooxygenase, Cytochrome P450 subfamily XXVIIB polypeptide 1, Cytochrome P450C1 alpha, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, CYP27B1, CYP1ALPHA, CYP27B |
| Target/Specificity | This CYP27B1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 477-508 amino acids from the C-terminal region of human CYP27B1. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CYP27B1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CYP27B1 |
|---|---|
| Synonyms | CYP1ALPHA, CYP27B |
| Function | A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1alpha- position to form the hormonally active form of vitamin D3, 1alpha,25- dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR) (PubMed:10518789, PubMed:10566658, PubMed:12050193, PubMed:22862690, PubMed:9486994). Has 1alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway (PubMed:10518789, PubMed:22862690). Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2 (PubMed:10518789). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed:22862690). |
| Cellular Location | Mitochondrion membrane. |
| Tissue Location | Kidney. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
REFERENCES
Fu G.K.,et al.DNA Cell Biol. 16:1499-1507(1997).
Monkawa T.,et al.Biochem. Biophys. Res. Commun. 239:527-533(1997).
Fu G.K.,et al.Mol. Endocrinol. 11:1961-1970(1997).
Huang D.C.,et al.Mol. Cancer Res. 1:56-67(2002).
Huang D.C.,et al.Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
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