- 文献引用 : 3
|Application ||WB, IHC-P, FC, E|
|Other Names||25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, VD3 1A hydroxylase, Calcidiol 1-monooxygenase, Cytochrome P450 subfamily XXVIIB polypeptide 1, Cytochrome P450C1 alpha, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, CYP27B1, CYP1ALPHA, CYP27B|
|Target/Specificity||This CYP27B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-508 amino acids from the C-terminal region of human CYP27B1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CYP27B1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D3) to 1-alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)(2)D3), and of 24,25-dihydroxyvitamin D3 (24,25(OH)(2)D3) to 1- alpha,24,25-trihydroxyvitamin D3 (1alpha,24,25(OH)(3)D3). Is also active with 25-hydroxy-24-oxo-vitamin D3. Plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.|
|Cellular Location||Mitochondrion membrane.|
Author : Chiang KC1, Yeh CN2, Huang CC3, Yeh TS4, S Pang JH5, Hsu JT6, Chen LW7, Kuo SF8, Kittaka A9, Chen TC10, Juang HH11.
Int J Mol Sci. 2016 Aug 12;17(8). pii: E1326. doi: 10.3390/ijms17081326.
Author : Schlosser RJ1,2, Carroll WW1, Soler ZM1, Pasquini WN1, Mulligan JK1,2,3.
Int Forum Allergy Rhinol. 2015 Nov 17. doi: 10.1002/alr.21576. [Epub ahead of print]
Author : Mandell E1, Seedorf GJ2, Ryan S3, Gien J3, Cramer SD4, Abman SH2.
Am J Physiol Lung Cell Mol Physiol. 2015 Nov 1;309(9):L1018-26. doi: 10.1152/ajplung.00253.2015. Epub 2015 Sep 4.
Provided below are standard protocols that you may find useful for product applications.
CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.
Simon,K.C., et.al., Mult. Scler. 16 (2), 133-138 (2010)
Zhou,S., et.al., Endocrinology 151 (1), 14-22 (2010)