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CYP27B1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

Anti-CYP27B1 Antibody (C-term) at 1:2000 dilution + Rat kidney whole tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing Hela cells stained with AP9056b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9056b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
CYP27B1 Antibody (C-term) (Cat. #AP9056B)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP27B1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

产品详情
文献引用 : 3
实验流程
背景知识

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	O15528
Reactivity	Human, Rat
Predicted	Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig

Additional info
Gene ID	1594
Other Names	25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, VD3 1A hydroxylase, Calcidiol 1-monooxygenase, Cytochrome P450 subfamily XXVIIB polypeptide 1, Cytochrome P450C1 alpha, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, CYP27B1, CYP1ALPHA, CYP27B
Target/Specificity	This CYP27B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-508 amino acids from the C-terminal region of human CYP27B1.
Dilution	WB~~1:2000 FC~~1:25 IHC-P~~1:10~50
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CYP27B1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CYP27B1
Synonyms	CYP1ALPHA, CYP27B
Function	Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D3) to 1-alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)(2)D3), and of 24,25-dihydroxyvitamin D3 (24,25(OH)(2)D3) to 1- alpha,24,25-trihydroxyvitamin D3 (1alpha,24,25(OH)(3)D3). Is also active with 25-hydroxy-24-oxo-vitamin D3. Plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
Cellular Location	Mitochondrion membrane.
Tissue Location	Kidney.

Research Areas

25(OH)D Is Effective to Repress Human Cholangiocarcinoma Cell Growth through the Conversion of 25(OH)D to 1α,25(OH)₂D₃.
Author : Chiang KC¹, Yeh CN², Huang CC³, Yeh TS⁴, S Pang JH⁵, Hsu JT⁶, Chen LW⁷, Kuo SF⁸, Kittaka A⁹, Chen TC¹⁰, Juang HH¹¹. Int J Mol Sci. 2016 Aug 12;17(8). pii: E1326. doi: 10.3390/ijms17081326.	27529229

Reduced sinonasal levels of 1α-hydroxylase are associated with worse quality of life in chronic rhinosinusitis with nasal polyps.
Author : Schlosser RJ^1,², Carroll WW¹, Soler ZM¹, Pasquini WN¹, Mulligan JK^1,^2,³. Int Forum Allergy Rhinol. 2015 Nov 17. doi: 10.1002/alr.21576. [Epub ahead of print]	26575398

Antenatal endotoxin disrupts lung vitamin D receptor and 25-hydroxyvitamin D 1α-hydroxylase expression in the developing rat.
Author : Mandell E¹, Seedorf GJ², Ryan S³, Gien J³, Cramer SD⁴, Abman SH². Am J Physiol Lung Cell Mol Physiol. 2015 Nov 1;309(9):L1018-26. doi: 10.1152/ajplung.00253.2015. Epub 2015 Sep 4.	26342089

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

REFERENCES

Simon,K.C., et.al., Mult. Scler. 16 (2), 133-138 (2010)
Zhou,S., et.al., Endocrinology 151 (1), 14-22 (2010)

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¥ 1,100.00

Cat# AP9056B

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CYP27B1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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