RAB27B Antibody

Purified Mouse Monoclonal Antibody (Mab)

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Overlay histogram showing A431 cells stained with AM8688b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8688b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-RAB27B Antibody at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: mouse stomach lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	O00194
Reactivity	Human, Mouse
Host	Mouse
Clonality	monoclonal
Isotype	IgG1,k
Clone Names	1596CT245.254.49
Calculated MW	24608 Da

Additional Information
Gene ID	5874
Other Names	Ras-related protein Rab-27B, C25KG, RAB27B
Target/Specificity	This RAB27B antibody is generated from a mouse immunized with a recombinant protein of human RAB27B.
Dilution	WB~~1:2000 FC~~1:25 E~~Use at an assay dependent concentration.
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RAB27B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	RAB27B
Function	Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed:30771381). Plays a role in NTRK2/TRKB axonal anterograde transport by facilitating the association of NTRK2/TRKB with KLC1 (PubMed:21775604). May be involved in targeting uroplakins to urothelial apical membranes (By similarity).
Cellular Location	Membrane; Lipid-anchor. Late endosome
Tissue Location	Expressed primarily in testis.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

May be involved in targeting uroplakins to urothelial apical membranes.

REFERENCES

Chen D.,et al.Biochem. Mol. Med. 60:27-37(1997).
Chen D.,et al.Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
Ramalho J.S.,et al.BMC Genet. 2:2-2(2001).
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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¥ 1,250.00

Cat# AM8688b

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¥ 1,250.00

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RAB27B Antibody

Purified Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

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