ISL1 Antibody

Mouse Monoclonal Antibody to ISL1

Figure 1: Western blot analysis using ISL1 mouse mAb against full-length ISL1 (aa1-349)-hIgGFc transfected HEK293 cell lysate(1).
Figure 2: Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using ISL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using anti-ISL1 monoclonal antioby (green). Blue: DRAQ5 fluorescent DNA dye.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, ICC, E
Primary Accession	P61371
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	1B1
Isotype	Mouse IgG1
Calculated MW	39036 Da
Description	ISL1 (ISL1 transcription factor, LIM/homeodomain) is a member of the LIM/homeodomain family of transcription factors. It binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. It is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Islet-1 expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry. Mutations in this gene have been associated with maturity-onset diabetes of the young.
Immunogen	Purified recombinant fragment of human ISL1 expressed in E. Coli.

Additional Information
Other Names	Insulin gene enhancer protein ISL-1, Islet-1, ISL1
Target/Specificity	Purified recombinant fragment of human ISL1 expressed in E. Coli.
Dilution	WB~~1:500~~2000 ICC~~1:200~~1000 E~~N/A
Format	Ascitic fluid containing 0.03% sodium azide.
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ISL1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.