human IgG (Fc Specific) Antibody

Purified Mouse Monoclonal Antibody

Figure 1: Western blot analysis using human IgG (Fc specific) mouse mAb against human serum (1).
Figure 1: Confocal immunofluorescence analysis of HL60 cells using CD18 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 1: Confocal immunofluorescence analysis of HL60 cells using anti-CD18 monoclonal antioby (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Confocal immunofluorescence analysis of BCBL-1 cells (left) and L1210 cells (right) using anti-CD18 monoclonal antioby (red). Blue: DRAQ5 fluorescent DNA dye.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2E4
Isotype	IgG1
Description	Specificity of antibody has been tested using Western blot, immunofluorescence, and ELISA. The product reacts with human IgG (Fc) with no significant reactivity with human IgG (Fab2), IgM or other serum proteins. We didn't test crossing with other species IgG (Fc).
Immunogen	Purified fusion protein with human IgG (Fc Specific) tag.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Dilution	WB~~1/500 - 1/2000 E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	human IgG (Fc Specific) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.