NKX2.5 Antibody

Purified Mouse Monoclonal Antibody

Figure 1: Western blot analysis using NKX2.5 mouse mAb against full-length NKX2.5 (aa1-324)-hIgGFc transfected HEK293 cell lysate (1).
Figure 4: Flow cytometric analysis of Hela cells using JAK3 mouse mAb (blue) and negative control (red).
Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using anti-JAK3 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Confocal immunofluorescence analysis of 3T3-L1 cells using anti-JAK3 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	P52952
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2E1
Isotype	IgG1
Calculated MW	34918 Da
Description	NKX2.5: NK2 transcription factor related, locus 5 (Drosophila), also known as CSX. It is a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants.
Immunogen	Purified recombinant fragment of human NKX2.5 expressed in E. Coli.
Formulation	Antibody are purified by protein G affinity chromatography. Liquid in PBS containing 0.03% sodium azide.

Additional Information
Gene ID	1482
Other Names	Homeobox protein Nkx-2.5, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E, NKX2-5, CSX, NKX2.5, NKX2E
Dilution	WB~~1/500 - 1/2000 E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	NKX2.5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	NKX2-5
Synonyms	CSX, NKX2.5, NKX2E
Function	Transcription factor required for the development of the heart and the spleen (PubMed:22560297). During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4 (By similarity). May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal (By similarity). Binds to the core DNA motif of NPPA promoter (PubMed:22849347, PubMed:26926761). Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression (PubMed:22560297). Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia (PubMed:29899023).
Cellular Location	Nucleus.
Tissue Location	Expressed only in the heart.