AKT2 Antibody

Purified Mouse Monoclonal Antibody

Figure 1: Western blot analysis using AKT2 mouse mAb against A431 (1), Jurkat (2), HEK293 (3), A549 (4), MCF-7 (5) and PC-12 (6) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded human Thymus tissues using anti-AKT2 mAb
Figure 3: Immunofluorescence analysis of PANC-1 (left) and Hela (right) cells using AKT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P31751
Reactivity	Human, Rat, Monkey
Host	Mouse
Clonality	Monoclonal
Clone Names	1B6
Isotype	IgG2b
Calculated MW	55769 Da
Description	Akt2 (also designated protein kinase B beta or v-akt murine thymoma viral oncogene homolog 2 ), with 481-amino acid protein (about 53kDa), belongs to the AKT serine/threonine protein kinase family, which also includes Akt1 and Akt3. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. Among the members of AKT family, Akt2 is associated with the development of human cancers. Akt2 inhibits cisplatin-induced JNK/p38 and Bax activation through phosphorylation of ASK1 and thus, plays an important role in chemoresistance. Further, Akt2 plays a specific role in muscle differentiation.
Immunogen	Purified recombinant fragment of human AKT2 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Other Names	RAC-beta serine/threonine-protein kinase, 2.7.11.1, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, AKT2
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 ICC~~N/A E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	AKT2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.