GOT2 Antibody

Purified Mouse Monoclonal Antibody

Figure 1: Western blot analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.
Figure 2: Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Immunohistochemical analysis of paraffin-embedded human Testis tissues using anti-CDC25C mouse mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, ICC, E
Primary Accession	P00505
Reactivity	Human, Mouse, Rat, Monkey
Host	Mouse
Clonality	Monoclonal
Clone Names	3E9
Isotype	IgG1
Calculated MW	47518 Da
Description	Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic andinner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and theurea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Immunogen	Purified recombinant fragment of human GOT2 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	2806
Other Names	Aspartate aminotransferase, mitochondrial, mAspAT, 2.6.1.1, 2.6.1.7, Fatty acid-binding protein, FABP-1, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, FABPpm, Transaminase A, GOT2
Dilution	WB~~1/500 - 1/2000 ICC~~N/A E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GOT2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GOT2 (HGNC:4433)
Function	Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
Cellular Location	Mitochondrion matrix. Cell membrane. Note=Exposure to alcohol promotes translocation to the cell membrane.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.