CDC25C Antibody

Purified Mouse Monoclonal Antibody

Figure 1: Western blot analysis using anti-CDC25C mAb against Hela (1), K562 (2), PC-3 (3), HEK293 (4) and Raw264.7 (5) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded human Testis tissues using anti-CDC25C mouse mAb
Figure 3: Flow cytometric analysis of PC-2 cells using CDC2 mouse mAb (green) and negative control (purple).
Figure 2: Immunofluorescence analysis of Hela cells using CDC2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2:Immunofluorescence analysis of Hela cells using CDC2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	P30307
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	1F12
Isotype	IgG1
Calculated MW	53365 Da
Description	Cdc25C is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It has been highly conserved during evolution and it plays a key role in the regulation of cell division. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Cdc25C is mainly expressed in G2 phase. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
Immunogen	Purified recombinant fragment of human CDC25C expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	995
Other Names	M-phase inducer phosphatase 3, 3.1.3.48, Dual specificity phosphatase Cdc25C, CDC25C
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CDC25C Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CDC25C
Function	Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed:8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed:8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed:8119945).
Cellular Location	Nucleus

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.