ACTA2 Antibody

Purified Mouse Monoclonal Antibody

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);
Figure 1: Western blot analysis using ACTA2 mouse mAb against Hela (1), and Cos7 (2) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded liver tissues (left) and lung cancer tissues (right) using ACTA2 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded stomach cancer (left) and stomach tissues (right) using ACTA2 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	P62736
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Clone Names	4F4
Isotype	IgG1
Calculated MW	42009 Da
Description	Actin, alpha 2, smooth muscle, aorta, major constituent of thin filaments.
Immunogen	Purified recombinant fragment of human ACTA2 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	59
Other Names	Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ACTA2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ACTA2
Synonyms	ACTSA, ACTVS
Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location	Cytoplasm, cytoskeleton.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.