BECN1 Antibody

Purified Mouse Monoclonal Antibody

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);
Figure 1: Western blot analysis using BECN1 mouse mAb against Hela (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and liver cancer tissues (right) using BECN1 mouse mAb with DAB staining.
Figure 3: Flow cytometric analysis of RAJI cells using BECN1 mouse mAb (green) and negative control (purple).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	Q14457
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2A4
Isotype	IgG1
Calculated MW	51896 Da
Description	Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 (PubMed 19270693)).(supplied by OMIM) . Tissue specificity: Ubiquitous.
Immunogen	Purified recombinant fragment of human BECN1 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	8678
Other Names	Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197
Dilution	WB~~1/500 - 1/2000 IHC~~1/500 - 1/2000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	BECN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	BECN1
Synonyms	GT197
Function	Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense.
Cellular Location	Cytoplasm. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Endosome {ECO:0000250\|UniProtKB:O88597} Cytoplasmic vesicle, autophagosome. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity) {ECO:0000250\|UniProtKB:O88597, ECO:0000269\|PubMed:19050071} [Beclin-1-C 37 kDa]: Mitochondrion {ECO:0000250\|UniProtKB:O88597}
Tissue Location	Ubiquitous.