PRK2 Antibody

Purified Mouse Monoclonal Antibody

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);
Figure 1: Western blot analysis using PRK2 mAb against human PRK2 (AA: 555-718) recombinant protein. (Expected MW is 43.9 kDa)
Figure 2: Western blot analysis using PRK2 mouse mAb against PC-12 (1), Cos7 (2), K562 (3), Jurkat (4), Hela (5), A431 (6), C6 (7), NIH/3T3 (8) and HEK293 (9) cell lysate.
Figure 3: Immunohistochemical analysis of paraffin-embedded prostate tissues using PRK2 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PRK2 mouse mAb with DAB staining.
Figure 5: Flow cytometric analysis of NIH/3T3 cells using PRK2 mouse mAb (blue) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	Q16513
Reactivity	Human, Mouse, Rat, Monkey
Host	Mouse
Clonality	Monoclonal
Clone Names	1D1
Isotype	IgG1
Calculated MW	112035 Da
Description	Protein-kinase-C-related kinases (PRKs) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN. Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis.
Immunogen	Purified recombinant fragment of human PRK2 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Other Names	Serine/threonine-protein kinase N2, 2.7.11.13, PKN gamma, Protein kinase C-like 2, Protein-kinase C-related kinase 2, PKN2, PRK2, PRKCL2
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PRK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.