AlCAM Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using AlCAM mAb against human AlCAM recombinant protein. (Expected MW is 26.3 kDa)
Figure 2: Western blot analysis using AlCAM mouse mAb against L1210 (1) cell lysate, and Mouse spleen (2) tissue lysate.
Figure 3: Immunohistochemical analysis of paraffin-embedded striated muscle tissues using AlCAM mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	Q13740
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	5B3
Isotype	IgG1
Calculated MW	65102 Da
Description	This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
Immunogen	Purified recombinant fragment of human AlCAM (AA: 405-524) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Other Names	CD166 antigen, Activated leukocyte cell adhesion molecule, CD166, ALCAM, MEMD
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	AlCAM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.