ONECUT3 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using ONECUT3 mAb against human ONECUT3 recombinant protein. (Expected MW is 38.2 kDa)
Figure 2: Western blot analysis using ONECUT3 mAb against HEK293 (1) and ONECUT3 (AA: 350-455)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ONECUT3 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ONECUT3 mouse mAb with DAB staining.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	O60422
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	10B2F5
Isotype	IgG1
Calculated MW	50037 Da
Description	The protein encoded by this gene is a transcriptional activator. It can bind the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR(By similarity).
Immunogen	Purified recombinant fragment of human ONECUT3 (AA: 350-455) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	390874
Other Names	One cut domain family member 3, One cut homeobox 3, Transcription factor ONECUT-3, OC-3, ONECUT3
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ONECUT3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ONECUT3
Function	Transcriptional activator. Binds the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR (By similarity).
Cellular Location	Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00108, ECO:0000255\|PROSITE-ProRule:PRU00374}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). ;

REFERENCES

1. Biochem Biophys Res Commun. 2002 Apr 12;292(4):848-54.

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¥ 2,600.00

Cat# AO1836a

Size:

100 µl

Price:

¥ 2,600.00

Quantity:

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Availability: 3-5 days

询价

ONECUT3 Antibody

Purified Mouse Monoclonal Antibody

BACKGROUND

REFERENCES

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