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ONECUT3 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ONECUT3 Antibody (C-term) AP18070b
    Anti-ONECUT3 Antibody (C-term) at 1:1000 dilution + MDA-MB-231 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession O60422
Other Accession Q8K557, NP_001073957.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 50037 Da
Antigen Region 393-419 aa
Additional Information
Gene ID 390874
Other Names One cut domain family member 3, One cut homeobox 3, Transcription factor ONECUT-3, OC-3, ONECUT3
Target/Specificity This ONECUT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-419 amino acids from the C-terminal region of human ONECUT3.
Dilution WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsONECUT3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ONECUT3
Function Transcriptional activator. Binds the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE-ProRule:PRU00374}
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

ONECUT3 is transcriptional activator. It binds the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR (By similarity).

REFERENCES

Grimwood, J., et al. Nature 428(6982):529-535(2004)
Vanhorenbeeck, V., et al. Biochem. Biophys. Res. Commun. 292(4):848-854(2002)
Venter, J.C., et al. Science 291(5507):1304-1351(2001)
Jacquemin, P., et al. J. Biol. Chem. 274(5):2665-2671(1999)

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