EIF5A Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using EIF5A mAb against human EIF5A(AA: 1-154) recombinant protein. (Expected MW is 42.8 kDa)
Figure 2: Western blot analysis using EIF5A mAb against HEK293 (1) and EIF5A (AA: 1-154)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Flow cytometric analysis of Hela cells using EIF5A mouse mAb (green) and negative control (red).
Figure 4: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF5A mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	P63241
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	4E10F6
Isotype	IgG1
Calculated MW	16832 Da
Description	EIF5A (eukaryotic translation initiation factor 5A) is a protein-coding gene. Diseases associated with EIF5A include lung adenocarcinoma, and intrahepatic cholangiocarcinoma, and among its related super-pathways are Post-translational protein modification and Apoptotic Pathways in Synovial Fibroblasts. GO annotations related to this gene include ribosome binding and RNA binding. An important paralog of this gene is EIF5AL1.
Immunogen	Purified recombinant fragment of human EIF5A (AA:1-154) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Other Names	Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	EIF5A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.