KBTBD8 Antibody

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using KBTBD8 mAb against human KBTBD8 (AA: 264-464) recombinant protein. (Expected MW is 48.7 kDa)
Figure 3:Western blot analysis using KBTBD8 mAb =against HEK293 (1) and KBTBD8 (AA: 264-464)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using KBTBD8 mouse mAb against A431 (1), Jurkat (2), Hela (3), K562 (4), and HEK293 (5) cell lysate.
Figure 5:Flow cytometric analysis of A431 cells using KBTBD8 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q8NFY9
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7G8A6
Isotype	IgG1
Calculated MW	68823 Da
Description	KBTBD8 (kelch repeat and BTB (POZ) domain containing 8) is a protein-coding gene. An important paralog of this gene is KLHL21.
Immunogen	Purified recombinant fragment of human KBTBD8 (AA: 264-464) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	84541
Other Names	Kelch repeat and BTB domain-containing protein 8, T-cell activation kelch repeat protein, TA-KRP, KBTBD8, KIAA1842, TAKRP
Dilution	WB~~1/500 - 1/2000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	KBTBD8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	KBTBD8
Synonyms	KIAA1842, TAKRP
Function	Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of neural crest specification (PubMed:26399832). The BCR(KBTBD8) complex acts by mediating monoubiquitination of NOLC1 and TCOF1: monoubiquitination promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832).
Cellular Location	Cytoplasm, cytoskeleton, spindle. Golgi apparatus. Note=Translocates to the spindle apparatus during mitosis.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.