P2RY13 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using P2RY13 mAb against human P2RY13 (AA: 1–49) recombinant protein. (Expected MW is 31.6 kDa)
Figure 3:Western blot analysis using P2RY13 mAb against HEK293 (1) and P2RY13 (AA: 1–49)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of Hela cells using P2RY13 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q9BPV8
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3E8C12
Isotype	IgG1
Calculated MW	40789 Da
Description	The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is activated by ADP.
Immunogen	Purified recombinant fragment of human P2RY13 (AA: 1–49) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	53829
Other Names	P2Y purinoceptor 13, P2Y13, G-protein coupled receptor 86, G-protein coupled receptor 94, P2RY13, GPR86, GPR94
Dilution	WB~~1/500 - 1/2000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	P2RY13 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	P2RY13
Synonyms	GPR86, GPR94
Function	Receptor for ADP. Coupled to G(i)-proteins. May play a role in hematopoiesis and the immune system.
Cellular Location	Cell membrane; Multi-pass membrane protein.
Tissue Location	Strong expression in spleen and adult brain. Lower expression in placenta, lung, liver, spinal cord, thymus, small intestine, uterus, stomach, testis, fetal brain, and adrenal gland. Not detected in pancreas, heart, kidney, skeletal muscle, ovary or fetal aorta. Clearly detected in lymph node and bone marrow, weakly detected in peripheral blood mononuclear cells (PBMC) and in peripheral blood leukocytes (PBL), but not detected in polymorphonuclear cells (PMN). In the brain, detected in all brain regions examined

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.