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Anti-GPR86 Antibody

Rabbit polyclonal antibody to GPR86

     
  • 1 - Anti-GPR86 Antibody AP60602
    Western blot analysis of GPR86 expression in HEK293T (A), Hela (B), HepG2 (C) whole cell lysates.
  • 3 - Anti-GPR86 Antibody AP60602
    Immunofluorescent analysis of GPR86 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IP, IF/IC
Primary Accession Q9BPV8
Other Accession Q9D8I2
Reactivity Human, Mouse, Rat, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW 40789 Da
Additional Information
Gene ID 53829
Other Names GPR86; GPR94; P2Y purinoceptor 13; P2Y13; G-protein coupled receptor 86; G-protein coupled receptor 94
Target/Specificity Recognizes endogenous levels of GPR86 protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
IP~~N/A
IF/IC~~N/A
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name P2RY13
Synonyms GPR86, GPR94
Function Receptor for ADP. Coupled to G(i)-proteins. May play a role in hematopoiesis and the immune system.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Strong expression in spleen and adult brain. Lower expression in placenta, lung, liver, spinal cord, thymus, small intestine, uterus, stomach, testis, fetal brain, and adrenal gland. Not detected in pancreas, heart, kidney, skeletal muscle, ovary or fetal aorta. Clearly detected in lymph node and bone marrow, weakly detected in peripheral blood mononuclear cells (PBMC) and in peripheral blood leukocytes (PBL), but not detected in polymorphonuclear cells (PMN). In the brain, detected in all brain regions examined
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR86. The exact sequence is proprietary.

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