Anti-GPR86 Antibody

Rabbit polyclonal antibody to GPR86

Western blot analysis of GPR86 expression in HEK293T (A), Hela (B), HepG2 (C) whole cell lysates.
Immunofluorescent analysis of GPR86 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IP, IF/IC
Primary Accession	Q9BPV8
Other Accession	Q9D8I2
Reactivity	Human, Mouse, Rat, Monkey
Host	Rabbit
Clonality	Polyclonal
Calculated MW	40789 Da

Additional Information
Gene ID	53829
Other Names	GPR86; GPR94; P2Y purinoceptor 13; P2Y13; G-protein coupled receptor 86; G-protein coupled receptor 94
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR86. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100) IP~~N/A IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	P2RY13
Synonyms	GPR86, GPR94
Function	Receptor for ADP. Coupled to G(i)-proteins. May play a role in hematopoiesis and the immune system.
Cellular Location	Cell membrane; Multi-pass membrane protein.
Tissue Location	Strong expression in spleen and adult brain. Lower expression in placenta, lung, liver, spinal cord, thymus, small intestine, uterus, stomach, testis, fetal brain, and adrenal gland. Not detected in pancreas, heart, kidney, skeletal muscle, ovary or fetal aorta. Clearly detected in lymph node and bone marrow, weakly detected in peripheral blood mononuclear cells (PBMC) and in peripheral blood leukocytes (PBL), but not detected in polymorphonuclear cells (PMN). In the brain, detected in all brain regions examined

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.