LGR5 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using LGR5 mAb against human LGR5 (AA: 22-178) recombinant protein. (Expected MW is 43.4 kDa)
Figure 3:Western blot analysis using LGR5 mAb against HEK293 (1) and LGR5 (AA: 22-178)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of A549 cells using LGR5 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	O75473
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2B5B9
Isotype	IgG2b
Calculated MW	99998 Da
Description	LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5) is a Protein Coding gene. Among its related pathways are Wnt signaling pathway (KEGG). GO annotations related to this gene include G-protein coupled receptor activity and transmembrane signaling receptor activity. An important paralog of this gene is LGR6.
Immunogen	Purified recombinant fragment of human LGR5 (AA: 22-178) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Other Names	Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67
Dilution	WB~~1/500 - 1/2000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	LGR5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.