MECP2 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using MECP2 mAb against human MECP2 (AA: 7-148) recombinant protein. (Expected MW is 41.7 kDa)
Figure 3:Western blot analysis using MECP2 mAb against HEK293 (1) and MECP2 (AA: 7-148)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using MECP2 mouse mAb against A431 (1) and MCF-7 (2) cell lysate.
Figure 5:Immunofluorescence analysis of Hela cells using MECP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Flow cytometric analysis of Hela cells using MECP2 mouse mAb (green) and negative control (red).
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MECP2 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, ICC, E
Primary Accession	P51608
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	8H4A5B9
Isotype	IgG1
Calculated MW	52441 Da
Description	DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Immunogen	Purified recombinant fragment of human MECP2 (AA: 7-148) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	4204
Other Names	Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	MECP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	MECP2
Function	Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)- containing DNA, with a preference for 5-methylcytosine (5mC).
Cellular Location	Nucleus {ECO:0000250\|UniProtKB:Q9Z2D6}. Note=Colocalized with methyl-CpG in the genome. Colocalized with TBL1X to the heterochromatin foci.
Tissue Location	Present in all adult somatic tissues tested.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.