GUCY1A3 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using GUCY1A3 mAb against human GUCY1A3 recombinant protein. (Expected MW is 47.2 kDa)
Figure 2: Western blot analysis using GUCY1A3 mAb against HEK293 (1) and GUCY1A3 (AA: 22-214)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Western blot analysis using GUCY1A3 mouse mAb against HEK293 (1) and Jurkat (2) cell lysate.
Figure 4: Immunofluorescence analysis of HepG2 cells using GUCY1A3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 5: Flow cytometric analysis of HEK293 cells using GUCY1A3 mouse mAb (green) and negative control (purple).
Figure 6: Immunohistochemical analysis of paraffin-embedded renal cancer tissues using GUCY1A3 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, ICC, E
Primary Accession	Q02108
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3G6B2
Isotype	IgG1
Calculated MW	77452 Da
Description	Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene.
Immunogen	Purified recombinant fragment of human GUCY1A3 (AA: 22-214) expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	2982
Other Names	Guanylate cyclase soluble subunit alpha-3, GCS-alpha-3, 4.6.1.2, GCS-alpha-1, Soluble guanylate cyclase large subunit, GUCY1A3, GUC1A3, GUCSA3, GUCY1A1
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GUCY1A3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.