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Mouse Monoclonal Antibody to HLA-B

Purified Mouse Monoclonal Antibody

     
  • 10 - Mouse Monoclonal Antibody to HLA-B AO2443a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • 1 - Mouse Monoclonal Antibody to HLA-B AO2443a
    Western blot analysis using HLA-B mAb against human HLA-B (AA: 241-362) recombinant protein. (Expected MW is 38.4 kDa)
  • 1 - Mouse Monoclonal Antibody to HLA-B AO2443a
    Western blot analysis using HLA-B mAb against HEK293 (1) and HLA-B (AA: 241-362)-hIgGFc transfected HEK293 (2) cell lysate.
  • 1 - Mouse Monoclonal Antibody to HLA-B AO2443a
    Western blot analysis using HLA-B mouse mAb against Ramos (1) and A431 (2) cell lysate.
  • 8 - Mouse Monoclonal Antibody to HLA-B AO2443a
    Immunofluorescence analysis of Hela cells using HLA-B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • 8 - Mouse Monoclonal Antibody to HLA-B AO2443a
    Immunofluorescence analysis of Hela cells using HLA-B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
  • 4 - Mouse Monoclonal Antibody to HLA-B AO2443a
    Flow cytometric analysis of MCF-7 cells using HLA-B mouse mAb (green) and negative control (red).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, FC, ICC, E
Primary Accession P01889
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2G7B10
Isotype Mouse IgG1
Calculated MW 40460 Da
Description HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.;
Immunogen Purified recombinant fragment of human HLA-B (AA: 241-362) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Additional Information
Gene ID 3106
Other Names AS; HLAB; Bw-47; Bw-50; SPDA1; B-4901; B-5001; HLA-Cw;
Dilution WB~~1:1000
IHC~~1:100~500
FC~~1:10~50
ICC~~N/A
E~~N/A
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to HLA-B is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HLA-B (HGNC:4932)
Synonyms HLAB
Function Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-B-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells (PubMed:23209413, PubMed:25808313, PubMed:29531227, PubMed:9620674). May also present self-peptides derived from the signal sequence of secreted or membrane proteins, although T cells specific for these peptides are usually inactivated to prevent autoreactivity (PubMed:18991276, PubMed:7743181). Both the peptide and the MHC molecule are recognized by TCR, the peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells (PubMed:24600035, PubMed:29531227, PubMed:9620674). Typically presents intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via constitutive proteasome and IFNG-induced immunoproteasome (PubMed:23209413). Can bind different peptides containing allele-specific binding motifs, which are mainly defined by anchor residues at position 2 and 9 (PubMed:25808313, PubMed:29531227).
Cellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

1.Pharmacogenomics J. 2015 Oct;15(5):467-72. ; 2.J Immunol. 2014 Jun 1;192(11):4967-76.;

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