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Mouse Monoclonal Antibody to THBS1

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using THBS1 mAb against human THBS1 (AA: 750-850) recombinant protein. (Expected MW is 37.3 kDa)
Western blot analysis using THBS1 mAb against HEK293 (1) and THBS1 (AA: 750-850)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric analysis of Hela cells using THBS1 mouse mAb (green) and negative control (red).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	P07996
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	5E2E2
Isotype	Mouse IgG1
Calculated MW	129383 Da
Description	The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. ;
Immunogen	Purified recombinant fragment of human THBS1 (AA: 750-850) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide
Application Note	ELISA: 1/10000; WB: 1/500 - 1/2000; FCM: 1/200 - 1/400

Additional Information
Gene ID	7057
Other Names	TSP; THBS; TSP1; TSP-1; THBS-1
Dilution	WB~~1:1000 FC~~1:10~50 E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Mouse Monoclonal Antibody to THBS1 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	THBS1 (HGNC:11785)
Synonyms	TSP, TSP1
Function	Adhesive glycoprotein that mediates cell-to-cell and cell-to- matrix interactions (PubMed:15014436, PubMed:18285447, PubMed:2430973, PubMed:6489349). Multifunctional, involved in inflammation, angiogenesis, wound healing, reactive oxygen species (ROS) signaling, nitrous oxide (NO) signaling, apoptosis, senescence, aging, cellular self-renewal, stemness, and cardiovascular and metabolic homeostasis (PubMed:10613822, PubMed:11134179, PubMed:1371676, PubMed:14568985, PubMed:24511121, PubMed:29042481, PubMed:32679764). Negatively modulates dendritic cell activation and cytokine release, as part of an autocrine feedback loop, contributing to the resolution of inflammation and immune homeostasis (PubMed:14568985). Ligand for receptor CD47 (PubMed:19004835, PubMed:8550562). Modulates nitrous oxide (NO) signaling via CD47, hence playing a role as a pressor agent, supporting blood pressure (By similarity). Plays a role in endothelial cell senescence, acting via CD47, by increasing the abundance and activation of NADPH oxidase NOX1, and so generating excess ROS (PubMed:29042481). Inhibits stem cell self-renewal, acting via CD47 signaling, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (By similarity). Negatively modulates wound healing, acting via CD47 (By similarity). Ligand for receptor CD36 (PubMed:10613822, PubMed:11134179, PubMed:1371676). Involved in inducing apoptosis in podocytes in response to elevated free fatty acids, acting via CD36 (By similarity). Plays a role in suppressing angiogenesis, acting, depending on context, via CD36 or CD47 (PubMed:10613822, PubMed:11134179, PubMed:1371676, PubMed:32679764). Promotes cellular senescence in a TP53-CDKN1A-RB1 signaling-dependent manner (PubMed:29042481). Ligand for immunoglobulin-like cell surface receptor SIRPA (PubMed:24511121). Involved in ROS signaling in non- phagocytic cells, stimulating NADPH oxidase-derived ROS production, acting via interaction with SIRPA (PubMed:24511121). Plays a role in metabolic dysfunction in diet-induced obesity, perhaps acting by exacerbating adipose inflammatory activity; its effects may be mediated, at least in part, through enhanced adipocyte proliferation (By similarity). Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity). May be involved in age-related conditions, including metabolic dysregulation, during normal aging (PubMed:29042481, PubMed:32679764).
Cellular Location	Secreted. Cell surface. Secreted, extracellular space, extracellular matrix. Endoplasmic reticulum {ECO:0000250\|UniProtKB:P35441}. Sarcoplasmic reticulum {ECO:0000250\|UniProtKB:P35441}. Note=Secreted by thrombin-activated platelets and binds to the cell surface in the presence of extracellular Ca(2+) (PubMed:101549, PubMed:6777381). Incorporated into the extracellular matrix (ECM) of fibroblasts (PubMed:6341993). The C- terminal region in trimeric form is required for retention in the ECM (PubMed:18285447). Also detected in the endoplasmic reticulum and sarcoplasmic reticulum where it plays a role in the ER stress response (By similarity). {ECO:0000250\|UniProtKB:P35441, ECO:0000269\|PubMed:6341993, ECO:0000269\|PubMed:6777381}
Tissue Location	Expressed by platelets (at protein level) (PubMed:101549). Expressed by monocyte-derived immature and mature dendritic cells (at protein level) (PubMed:14568985)