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>   首页   >   产品   >   一抗   >   癌症   >   THBS1 Antibody (N-term)   

THBS1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - THBS1 Antibody (N-term) AP8522A
    Western blot analysis of THBS1 Antibody (N-term) (Cat. #AP8522a) in MDA-MB231 cell line lysates (35ug/lane). THBS1 (arrow) was detected using the purified Pab.
  • 14 - THBS1 Antibody (N-term) AP8522A
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with THBS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - THBS1 Antibody (N-term) AP8522A
    Confocal immunofluorescent analysis of THBS1 Antibody (N-term) (Cat#AP8522a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • 4 - THBS1 Antibody (N-term) AP8522A
    THBS1 Antibody (N-term) (Cat. #AP8522a) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, FC, E
Primary Accession P07996
Other Accession Q28178
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 129383 Da
Additional Information
Gene ID 7057
Other Names Thrombospondin-1, THBS1, TSP, TSP1
Target/Specificity This THBS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-210 amino acids from the N-terminal region of human THBS1.
Dilution WB~~1:1000
IHC-P~~1:100~500
IF~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTHBS1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name THBS1
Synonyms TSP, TSP1
Function Adhesive glycoprotein that mediates cell-to-cell and cell-to- matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity).
Cellular Location Secreted. Cell surface. Secreted, extracellular space, extracellular matrix. Endoplasmic reticulum {ECO:0000250|UniProtKB:P35441}. Sarcoplasmic reticulum {ECO:0000250|UniProtKB:P35441}. Note=Secreted by thrombin-activated platelets and binds to the cell surface in the presence of extracellular Ca(2+) (PubMed:6777381). Incorporated into the extracellular matrix of fibroblasts (PubMed:6341993). Also detected in the endoplasmic reticulum and sarcoplasmic reticulum where it plays a role in the ER stress response (By similarity) {ECO:0000250|UniProtKB:P35441, ECO:0000269|PubMed:6341993, ECO:0000269|PubMed:6777381}
Research Areas

BACKGROUND

THBS1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis,and tumorigenesis.

REFERENCES

Hofsteenge,J., et.al., J. Biol. Chem. 276 (9), 6485-6498 (2001)
Roszmusz,E., et.al., Biochem. Biophys. Res. Commun. 296 (1), 156-160 (2002)

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