NAA10

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using NAA10 mAb against human NAA10 (AA: 111-235) recombinant protein. (Expected MW is 47.2 kDa)
Figure 3:Western blot analysis using NAA10 mAb against HEK293 (1) and NAA10 (AA: 111-235)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using NAA10 mouse mAb against HCT116 (1), COS7 (2), HEK293 (3), HL-60 (4), MCF-7 (5), Hela (6), NIH/3T3 (7), and C2C12 (8) cell lysate.
Figure 5:Immunofluorescence analysis of Hela cells using NAA10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P41227
Reactivity	Human, Mouse, Monkey
Host	Mouse
Clonality	Monoclonal
Clone Names	3G3E9
Isotype	Mouse IgG1
Calculated MW	26459 Da
Immunogen	Purified recombinant fragment of human NAA10 (AA: 111-235) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Other Names	TE2; ARD1; NATD; ARD1A; ARD1P; OGDNS; DXS707; MCOPS1
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~ 1/200 - 1/1000 E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	NAA10 is for research use only and not for use in diagnostic or therapeutic procedures.