GRM7

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using GRM7 mAb against human GRM7 (AA: 454-590) recombinant protein. (Expected MW is 41.7 kDa)
Figure 3:Western blot analysis using GRM7 mAb against HEK293 (1) and GRM7 (AA: 454-590)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 6:Flow cytometric analysis of SH-SY5Y cells using GRM7 mouse mAb (green) and negative control (red).
Figure 4:Immunofluorescence analysis of Hela cells using GRM7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of SMMC-7721 cells using GRM7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	Q14831
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7G8D3
Isotype	Mouse IgG1
Calculated MW	102251 Da
Immunogen	Purified recombinant fragment of human GRM7 (AA: 454-590) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	2917
Other Names	GLUR7; MGLU7; GPRC1G; MGLUR7; PPP1R87
Dilution	WB~~ 1/300 - 1/800 IHC~~1:100~500 ICC~~ 1/200 - 1/1000 E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GRM7 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GRM7
Synonyms	GPRC1G, MGLUR7
Function	G protein-coupled receptor activated by glutamate that regulates axon outgrowth through the MAPK-cAMP-PKA signaling pathway during neuronal development (PubMed:33500274). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide- binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase that it inhibits (PubMed:9473604).
Cellular Location	Cell membrane; Multi-pass membrane protein
Tissue Location	Expressed in many areas of the brain, especially in the cerebral cortex, hippocampus, and cerebellum. Expression of GRM7 isoforms in non-neuronal tissues appears to be restricted to isoform 3 and isoform 4.