ACVR1

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using ACVR1 mAb against human ACVR1 (AA: 21-120) recombinant protein. (Expected MW is 37.1 kDa)
Figure 3:Western blot analysis using ACVR1 mAb against HEK293 (1) and ACVR1 (AA: 21-120)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 6:Flow cytometric analysis of Hela cells using ACVR1 mouse mAb (green) and negative control (red).
Figure 4:Immunofluorescence analysis of Hela cells using ACVR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 5:Immunofluorescence analysis of Hela cells using ACVR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using ACVR1 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	Q04771
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2E2C11
Isotype	Mouse IgG1
Calculated MW	57153 Da
Immunogen	Purified recombinant fragment of human ACVR1 (AA: 21-120) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	90
Other Names	FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~ 1/200 - 1/1000 E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ACVR1 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ACVR1
Synonyms	ACVRLK2
Function	Bone morphogenetic protein (BMP) type I receptor that is involved in a wide variety of biological processes, including bone, heart, cartilage, nervous, and reproductive system development and regulation (PubMed:20628059, PubMed:22977237). As a type I receptor, forms heterotetrameric receptor complexes with the type II receptors AMHR2, ACVR2A or ACVR2B (PubMed:17911401). Upon binding of ligands such as BMP7 or GDF2/BMP9 to the heteromeric complexes, type II receptors transphosphorylate ACVR1 intracellular domain (PubMed:25354296). In turn, ACVR1 kinase domain is activated and subsequently phosphorylates SMAD1/5/8 proteins that transduce the signal (PubMed:9748228). In addition to its role in mediating BMP pathway-specific signaling, suppresses TGFbeta/activin pathway signaling by interfering with the binding of activin to its type II receptor (PubMed:17911401). Besides canonical SMAD signaling, can activate non-canonical pathways such as p38 mitogen-activated protein kinases/MAPKs (By similarity). May promote the expression of HAMP, potentially via its interaction with BMP6 (By similarity).
Cellular Location	Membrane; Single-pass type I membrane protein.
Tissue Location	Expressed in normal parenchymal cells, endothelial cells, fibroblasts and tumor-derived epithelial cells