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GRM3

Purified Mouse Monoclonal Antibody

     
  • 10 - GRM3 AO2585a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • 1 - GRM3 AO2585a
    Figure 2:Western blot analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)
  • 1 - GRM3 AO2585a
    Figure 3:Western blot analysis using GRM3 mAb against HEK293 (1) and GRM3 (AA: extra 433-576)-hIgGFc transfected HEK293 (2) cell lysate.
  • 51 - GRM3 AO2585a
    Figure 5:Flow cytometric analysis of SH-SY5Y cells using GRM3 mouse mAb (green) and negative control (red).
  • 8 - GRM3 AO2585a
    Figure 4:Immunofluorescence analysis of Hela cells using GRM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
  • 2 - GRM3 AO2585a
    Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using GRM3 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, ICC, E
Primary Accession Q14832
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7A5A6
Isotype Mouse IgG2a
Calculated MW 98879 Da
Immunogen Purified recombinant fragment of human GRM3 (AA: extra 433-576) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2913
Other Names GLUR3; mGlu3; GPRC1C; MGLUR3
Dilution WB~~ 1/500 - 1/2000
IHC~~ 1/200 - 1/1000
ICC~~ 1/200 - 1/1000
E~~ 1/10000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGRM3 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GRM3
Synonyms GPRC1C, MGLUR3
Function G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Detected in brain cortex, thalamus, subthalamic nucleus, substantia nigra, hypothalamus, hippocampus, corpus callosum, caudate nucleus and amygdala.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

1.Prog Neuropsychopharmacol Biol Psychiatry. 2015 Oct 1;62:14-21.2.Cell Mol Biol (Noisy-le-grand). 2014 Sep 6;60(2):42-9.

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