F2R

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using F2R mAb against human F2R (AA: 42-176) recombinant protein. (Expected MW is 41.3 kDa)
Figure 3:Western blot analysis using F2R mAb against HEK293 (1) and F2R(AA: 42-176)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using F2R mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P25116
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	5E2E6
Isotype	Mouse IgG1
Calculated MW	47441 Da
Immunogen	Purified recombinant fragment of human F2R (AA: 42-176) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	2149
Other Names	TR; HTR; CF2R; PAR1; PAR-1
Dilution	WB~~ 1/500 - 1/2000 IHC~~ 1/200 - 1/1000 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	F2R is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	F2R (HGNC:3537)
Synonyms	CF2R, PAR1, TR
Function	High affinity receptor that binds the activated thrombin, leading to calcium release from intracellular stores (PubMed:1672265, PubMed:8136362). The thrombin-activated receptor signaling pathway is mediated through PTX-insensitive G proteins, activation of phospholipase C resulting in the production of 1D-myo-inositol 1,4,5- trisphosphate (InsP3) which binds to InsP3 receptors causing calcium release from the stores (By similarity). In astrocytes, the calcium released into the cytosol allows the Ca(2+)-dependent release of L- glutamate into the synaptic cleft through BEST1, that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (By similarity). May play a role in platelets activation and in vascular development (PubMed:10079109). Mediates up- regulation of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, triggered by coagulation factor Xa (F10) in cardiac fibroblasts and umbilical vein endothelial cells (PubMed:30568593, PubMed:34831181).
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:P26824}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P26824}
Tissue Location	Platelets and vascular endothelial cells.