P2RY1

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using P2RY1 mAb against human P2RY1 (AA: extra mix) recombinant protein. (Expected MW is 13 kDa)
Figure 3:Western blot analysis using P2RY1 mAb against HEK293 (1) and P2RY1 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using P2RY1 mouse mAb against SPC-A-1 (1) and C6 (2) cell lysate.
Figure 5:Flow cytometric analysis of Hela cells using P2RY1 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of K562 cells using P2RY1 mouse mAb (green) and negative control (red).
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using P2RY1 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P47900
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Clone Names	4G5D6
Isotype	Mouse IgG2b
Calculated MW	42072 Da
Immunogen	Purified recombinant fragment of human P2RY1 (AA: extra mix) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	5028
Other Names	P2Y1
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	P2RY1 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	P2RY1
Function	Receptor for extracellular adenine nucleotides such as ADP (PubMed:25822790, PubMed:9038354, PubMed:9442040). In platelets, binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and ultimately platelet aggregation (PubMed:9442040).
Cellular Location	Cell membrane; Multi-pass membrane protein

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.