CD183

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD183 mAb against human CD183 (AA: extra mix) recombinant protein. (Expected MW is 38.3 kDa)
Figure 3:Western blot analysis using CD183 mAb against HEK293 (1) and CD183 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using CD183 mouse mAb against Hela (1) and L-02 (2) cell lysate.
Figure 5:Flow cytometric analysis of HL-60 cells using CD183 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Jurkat cells using CD183 mouse mAb (green) and negative control (red).
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD183 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P49682
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	5C10B3
Isotype	Mouse IgG1
Calculated MW	40660 Da
Immunogen	Purified recombinant fragment of human CD183 (AA: extra mix) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	2833
Other Names	CXCR3; GPR9; MigR; CD182; Mig-R; CKR-L2; CMKAR3; IP10-R
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD183 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CXCR3
Synonyms	GPR9
Function	[Isoform 1]: Receptor for the C-X-C chemokines CXCL9, CXCL10 and CXCL11, and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G- protein signaling pathway (PubMed:12782716). Probably promotes cell chemotaxis response. Upon activation by PF4, induces activated T- lymphocyte migration mediated via downstream Ras/extracellular signal- regulated kinase (ERK) signaling. [Isoform 3]: Mediates the activity of CXCL11.
Cellular Location	[Isoform 1]: Cell membrane; Multi-pass membrane protein
Tissue Location	Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. Isoform 2 is expressed in endothelial cells. Expressed in T-cells (at protein level).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.