APC2

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using APC2 mAb against human APC2 (AA: 2041-2181) recombinant protein. (Expected MW is 40.9 kDa)
Figure 3:Western blot analysis using APC2 mAb against HEK293 (1) and APC2 (AA: 2041-2181)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 5:Flow cytometric analysis of Hela cells using APC2 mouse mAb (green) and negative control (red).
Figure 4:Immunofluorescence analysis of Hela cells using APC2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using APC2 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using APC2 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	O95996
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3A2G2
Isotype	Mouse IgG1
Calculated MW	243949 Da
Immunogen	Purified recombinant fragment of human APC2 (AA: 2041-2181) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	10297
Other Names	APCL
Dilution	WB~~ 1/500 - 1/2000 IHC~~ 1/200 - 1/1000 ICC~~ 1/200 - 1/1000 E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	APC2 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	APC2 (HGNC:24036)
Synonyms	APCL
Function	Stabilizes microtubules and may regulate actin fiber dynamics through the activation of Rho family GTPases (PubMed:25753423). May also function in Wnt signaling by promoting the rapid degradation of CTNNB1 (PubMed:10021369, PubMed:11691822, PubMed:9823329).
Cellular Location	Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasm Cytoplasm, perinuclear region Note=Associated with actin filaments (PubMed:11691822, PubMed:25753423). Associated with microtubule network (PubMed:10644998, PubMed:11691822, PubMed:25753423).
Tissue Location	Widely expressed (at protein level). Specifically expressed in the CNS.