GRM8

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using GRM8 mAb against human GRM8 (AA: extra 440-583) recombinant protein. (Expected MW is 42.6 kDa)
Figure 3:Western blot analysis using GRM8 mAb against HEK293 (1) and GRM8 (AA: extra 440-583)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using GRM8 mouse mAb against C6 (1) cell lysate.
Figure 5:Flow cytometric analysis of SK-N-SH cells using GRM8 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	O00222
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	4C1C1
Isotype	Mouse IgG1
Calculated MW	101741 Da
Immunogen	Purified recombinant fragment of human GRM8 (AA: extra 440-583) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	2918
Other Names	GLUR8; mGlu8; GPRC1H; MGLUR8
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GRM8 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GRM8
Synonyms	GPRC1H, MGLUR8
Function	G protein-coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.
Cellular Location	Cell membrane; Multi-pass membrane protein.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.