CD319

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD319 mAb against human CD319 (AA: extra 23-226) recombinant protein. (Expected MW is 48.3 kDa)
Figure 3:Western blot analysis using CD319 mAb against HEK293 (1) and CD319 (AA: extra 23-226)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of Raji cells using CD319 mouse mAb (green) and negative control (red).
Figure 5:Immunohistochemical analysis of paraffin-embedded tonsil tissues using CD319 mouse mAb with DAB staining.
1/200 - 1/1000

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	Q9NQ25
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	5G5A4
Isotype	Mouse IgG1
Calculated MW	37421 Da
Immunogen	Purified recombinant fragment of human CD319 (AA: extra 23-226) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	57823
Other Names	SLAMF7; 19A; CS1; CRACC
Dilution	WB~~ 1/500 - 1/2000 IHC~~ 1/200 - 1/1000 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD319 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	SLAMF7
Synonyms	CS1
Function	Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (PubMed:11698418). Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K (PubMed:16339536). In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Also acts inhibitory in T-cells (By similarity). May play a role in lymphocyte adhesion (PubMed:11802771). In LPS-activated monocytes negatively regulates production of pro-inflammatory cytokines (PubMed:23695528).
Cellular Location	Membrane; Single-pass type I membrane protein.
Tissue Location	Expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK- cell line but not in promyelocytic, B-, or T-cell lines. Expressed in monocytes. Isoform 3 is expressed at much lower level than isoform 1