> 首页 > 产品 > 一抗 > 精选抗体 > 内参抗体 > ACTB Antibody

ACTB Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of lysates from A431, HepG2 cell line (from left to right), using ACTB Antibody(Cat. #AP10168a). AP10168a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
ACTB Antibody (Cat. #AP10168a) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
ACTB Antibody (Cat. #AP10168a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

产品详情
文献引用 : 4
实验流程
背景知识

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, FC, WB, E
Primary Accession	P60709
Other Accession	A2BDB0, P63259, P63260, P63261, Q5ZMQ2, P63258, P60711, Q6QAQ1, P60710, Q4R561, P60706, P60712, P53505, NP_001092.1
Reactivity	Human
Predicted	Mouse, Rat, Monkey, Pig, Chicken, Bovine, Xenopus
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig

Additional info
Gene ID	60
Other Names	Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
Target/Specificity	This ACTB antibody is generated from rabbits immunized with a recombinant protein from human ACTB.
Dilution	WB~~1:500-1:1000 IHC-P~~1:50~100 FC~~1:10~50
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ACTB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ACTB
Function	Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G- actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947).
Cellular Location	Cytoplasm, cytoskeleton Nucleus. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs

Research Areas

Cysteine-rich protein 61 regulates adipocyte differentiation from mesenchymal stem cells through mammalian target of rapamycin complex 1 and canonical Wnt signaling.
Author : Yang Y1,2,Qi Q1,2,Wang Y1,2,Shi Y1,2,Yang W1,2,Cen Y1,2,Zhu E1,2,Li X3,Chen D4,Wang B1,2. FASEB J. 2018 Jan 18:fj201700830RR. doi: 10.1096/fj.201700830RR. [Epub ahead of print]	29401606

Expression of pituitary tumor-transforming 2 in human glioblastoma cell lines and its role in glioblastoma tumorigenesis.
Author : Guo Y¹, Shao Y², Chen J³, Xu S¹, Zhang X⁴, Liu H⁴. Exp Ther Med. 2016 May;11(5):1847-1852. Epub 2016 Mar 15.	27168815

Layer 2/3 pyramidal cells in the medial prefrontal cortex moderate stress induced depressive behaviors.
Author : Shrestha P¹, Mousa A¹, Heintz N¹. Elife. 2015 Sep 15;4. doi: 10.7554/eLife.08752.	26371510

Effect of 5-caffeoylquinic acid on the NF-κB signaling pathway, peroxisome proliferator-activated receptor gamma 2, and macrophage infiltration in high-fat diet-fed Sprague-Dawley rat adipose tissue.
Author : Liu SL¹, Peng BJ, Zhong YL, Liu YL, Song Z, Wang Z. Food Funct. 2015 Jul 9. [Epub ahead of print]	26158223

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

REFERENCES

Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010) Ambrosino, C., et al. Mol. Cell Proteomics 9(6):1352-1367(2010) Yoo, Y., et al. Oncogene 29(2):263-272(2010) Boratynska, A., et al. Acta Virol. 54(1):41-48(2010) Dugina, V., et al. J. Cell. Sci. 122 (PT 16), 2980-2988 (2009) :

终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问，联系：0512-88856768 tech-china@abcepta.com.

我要评价

¥ 1,100.00

Cat# AP10168a

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,100.00

¥ 1,800.00

¥ 2,600.00

¥ 4,000.00

Quantity:

reduce add

Availability: 2-4 weeks

询价

ACTB Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

相关产品

多肽合成服务

多克隆抗体制备服务

单克隆抗体制备服务

抗体测序服务

蛋白表达服务

药物发现定制服务