EFHB Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

EFHB Antibody (N-term) (Cat. #AP10904a) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the EFHB antibody detected the EFHB protein (arrow).
EFHB antibody (N-term) (Cat. #AP10904a) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EFHB antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
EFHB Antibody (N-term) (Cat. #AP10904a) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q8N7U6
Other Accession	NP_653316.3
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	93802 Da
Antigen Region	1-30 aa

Additional Information
Gene ID	151651
Other Names	EF-hand domain-containing family member B, EFHB
Target/Specificity	This EFHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EFHB.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	EFHB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	EFHB (HGNC:26330)
Synonyms	CFAP21
Function	Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating (PubMed:36191189). Cytosolic sensor for calcium, modulates the interaction of STIM1 and ORAI1 upon store depletion and the activation of store-operated Ca(2+) entry (SOCE) and NFAT translocation from cytosol to nucleus (PubMed:30481768).
Cellular Location	Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm, cytoskeleton, flagellum axoneme {ECO:0000250\|UniProtKB:Q8CDU5}. Cytoplasm
Tissue Location	Expressed in airway epithelial cells.