LRRC45 Antibody (C-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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Western blot analysis of lysates from human blood plasma tissue (from left to right),using LRRC45 Antibody (C-term)(Cat. #AP11368b).AP11368b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
Immunofluorescent analysis of U251 cells, using LRRC45 Antibody (C-term) (Cat. #AP11368b). AP11368b was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
LRRC45 Antibody (C-term) (Cat. #AP11368b)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LRRC45 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
LRRC45 Antibody (C-term) (Cat. #AP11368b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF, FC, E
Primary Accession	Q96CN5
Other Accession	NP_659436.1
Reactivity	Human, Mouse
Predicted	Rat, Bovine
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	75951 Da
Antigen Region	615-643 aa

Additional Information
Gene ID	201255
Other Names	Leucine-rich repeat-containing protein 45, LRRC45
Target/Specificity	This LRRC45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 615-643 amino acids from the C-terminal region of human LRRC45.
Dilution	WB~~1:1000 IHC-P~~1:100~500 IF~~1:25 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	LRRC45 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	LRRC45
Function	Component of the proteinaceous fiber-like linker between two centrioles, required for centrosome cohesion (PubMed:24035387). Is probably involved in the early stages of ciliogenesis (PubMed:30131441, PubMed:39638757). Plays a role in the organization of centriolar satellites, likely by regulating the accumulation of centriolar satellite proteins, such as PCM1 and SSX2IP, at the centrosome (PubMed:30131441).
Cellular Location	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=Localizes to the proteinaceous linker between the proximal ends of the centrioles (PubMed:24035387) Also localized to the distal appendages of the mother centriole (PubMed:30131441).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.