METTL10 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes: Anti-METTL10 Antibody (N-term) at 1:500 dilution + Mouse Brain lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 28 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
METTL10 Antibody (N-term) (Cat. #AP11370a)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of METTL10 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
METTL10 Antibody (N-term) (Cat. #AP11370a) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q5JPI9
Other Accession	NP_997719.2
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	25904 Da
Antigen Region	29-57 aa

Additional Information
Gene ID	399818
Other Names	Methyltransferase-like protein 10, 211-, METTL10, C10orf138
Target/Specificity	This METTL10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of human METTL10.
Dilution	WB~~1:500 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	METTL10 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	EFMT2
Function	[Isoform 2]: Protein-lysine methyltransferase that selectively catalyzes the trimethylation of EEF1A at 'Lys-318'.
Cellular Location	Cytoplasm {ECO:0000255\|HAMAP-Rule:MF_03188, ECO:0000269\|PubMed:25144183}. Nucleus {ECO:0000255\|HAMAP-Rule:MF_03188, ECO:0000269\|PubMed:25144183}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.