CCDC36 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

标注"皇冠"的产品，是满足高质量标准的产品。
了解更多...

Western blot analysis of lysates from 293,Neuro-2a cell line and mouse liver tissue (from left to right),using CCDC36 Antibody (C-term)(Cat. #AP11750b).AP11750b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
CCDC36 Antibody (C-term) (Cat. #AP11750b)immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCDC36 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of CCDC36 Antibody (C-term)(Cat#AP11750b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IF, WB, E
Primary Accession	Q8IYA8
Other Accession	NP_001128669.1, NP_835467.2
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	66343 Da
Antigen Region	422-450 aa

Additional Information
Gene ID	339834
Other Names	Coiled-coil domain-containing protein 36, Cancer/testis antigen 74, CT74, CCDC36
Target/Specificity	This CCDC36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 422-450 amino acids from the C-terminal region of human CCDC36.
Dilution	IHC-P~~1:100~500 IF~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CCDC36 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	IHO1 {ECO:0000303\|PubMed:27723721, ECO:0000312\|HGNC:HGNC:27945}
Function	Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.
Cellular Location	Chromosome {ECO:0000250\|UniProtKB:Q6PDM4}. Note=Specifically localizes to unsynapsed chromosomal regions during meiosis. {ECO:0000250\|UniProtKB:Q6PDM4}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.