SH2D4B Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

SH2D4B Antibody (C-term) (Cat. #AP11902b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SH2D4B antibody detected the SH2D4B protein (arrow).
SH2D4B Antibody (C-term) (Cat. #AP11902b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SH2D4B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of SH2D4B Antibody (C-term)(Cat#AP11902b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF, E
Primary Accession	Q5SQS7
Other Accession	NP_997255.2
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	51232 Da
Antigen Region	374-402 aa

Additional Information
Gene ID	387694
Other Names	SH2 domain-containing protein 4B, SH2D4B
Target/Specificity	This SH2D4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-402 amino acids from the C-terminal region of human SH2D4B.
Dilution	WB~~1:1000 IHC-P~~1:100~500 IF~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	SH2D4B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.