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>   首页   >   产品   >   一抗   >   细胞生物学   >   DLL3 Antibody (C-term)   

DLL3 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 59 - DLL3 Antibody (C-term) AP12393b
    Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue using AP12393B performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 59 - DLL3 Antibody (C-term) AP12393b
    Immunohistochemical analysis of paraffin-embedded Human brain tissue using AP12393B performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - DLL3 Antibody (C-term) AP12393b
    All lanes : Anti-DLL3 Antibody (C-term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: Human brain lysate Lane 4: SH-SY5Y whole cell lysate Lane 5: U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - DLL3 Antibody (C-term) AP12393b
    Confocal immunofluorescent analysis of DLL3 Antibody (C-term)(Cat#AP12393b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, IF, E
Primary Accession Q9NYJ7
Other Accession NP_058637.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 64618 Da
Antigen Region 545-573 aa
Additional Information
Gene ID 10683
Other Names Delta-like protein 3, Drosophila Delta homolog 3, Delta3, DLL3
Target/Specificity This DLL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 545-573 amino acids from the C-terminal region of human DLL3.
Dilution IHC-P-Leica~~1:500
WB~~1:2000
IF~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDLL3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DLL3
Function Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq].

REFERENCES

Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Heuss, S.F., et al. Proc. Natl. Acad. Sci. U.S.A. 105(32):11212-11217(2008)
Maisenbacher, M.K., et al. Hum. Genet. 116(5):416-419(2005)
Whittock, N.V., et al. Clin. Genet. 66(1):67-72(2004)
Bonafe, L., et al. Clin. Genet. 64(1):28-35(2003)

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