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RPL23 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - RPL23 Antibody (Center) AP12578c
    RPL23 Antibody (Center) (Cat. #AP12578c) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the RPL23 antibody detected the RPL23 protein (arrow).
  • 14 - RPL23 Antibody (Center) AP12578c
    RPL23 Antibody (Center) (Cat. #AP12578c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPL23 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P62829
Other Accession P62832, P62831, P62830, Q6PC14, Q3T057, NP_000969.1
Reactivity Human, Rat, Mouse
Predicted Bovine, Zebrafish, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 14865 Da
Antigen Region 49-78 aa
Additional Information
Gene ID 9349
Other Names 60S ribosomal protein L23, 60S ribosomal protein L17, RPL23
Target/Specificity This RPL23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from the Central region of human RPL23.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPL23 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPL23
Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Cellular Location Cytoplasm.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL17 because the encoded protein shares amino acid identity with ribosomal protein L17 from Saccharomyces cerevisiae; however, its official symbol is RPL23. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

REFERENCES

Gehring, N.H., et al. Cell 137(3):536-548(2009) Wanzel, M., et al. Nat. Cell Biol. 10(9):1051-1061(2008) Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005) Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005) Jin, A., et al. Mol. Cell. Biol. 24(17):7669-7680(2004)

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