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>   首页   >   产品   >   一抗   >   精选抗体   >   Bcl-G BH3 Domain Antibody   

Bcl-G BH3 Domain Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Bcl-G BH3 Domain Antibody AP1304a
    Anti-Bcl-G-BH3 Antibodyat 1:2000 dilution + human lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Bcl-G BH3 Domain Antibody AP1304a
    Western blot analysis of anti-Bcl-G BH3 Domain Pab (Cat. #AP1304a) in K562 cell line lysates (35ug/lane). Bcl-G BH3 Domain (arrow) was detected using the purified Pab.
  • 1 - Bcl-G BH3 Domain Antibody AP1304a
    Cell lysates were subjected to Western blotting to determine the expression of Bcl-gS protein. The levels of alpha-tubulin were determined to assure equal loading.
  • 14 - Bcl-G BH3 Domain Antibody AP1304a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9BZR8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 36598 Da
Additional Information
Gene ID 79370
Other Names Apoptosis facilitator Bcl-2-like protein 14, Bcl2-L-14, Apoptosis regulator Bcl-G, BCL2L14, BCLG
Target/Specificity This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-229 amino acids from human Bcl.
Dilution WB~~1:1000
IHC-P~~1:100~500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBcl-G BH3 Domain Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCL2L14
Synonyms BCLG
Function Plays a role in apoptosis.
Cellular Location Cytoplasm. [Isoform 2]: Endomembrane system. Note=Predominantly localized to cytosolic organelles
Tissue Location Isoform 1 is widely expressed. Isoform 2 is testis- specific.
Research Areas

BACKGROUND

Bcl-G belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Overexpression of Bcl-G has been shown to induce apoptosis in cells.

REFERENCES

Ozalp, S.S., et al., Eur J Gynaecol Oncol 23(5):419-422 (2002).
Guo, B., et al., J. Biol. Chem. 276(4):2780-2785 (2001).

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